대한약국학회

Objective: Objective of this study was to evaluate whether the reduced glutathione (GSH) entrapped into Ginspo nanoparticle (a new solid lipid nanocarrier) was absorbed by oral route. Methods: Ginspo nanoparticle was prepared by hot-melt method. Physicochemical characteristics of the Ginspo nanoparticle were measured, and its absorption by oral route was also observed with Sprague-Dawley rats and a healthy human. Results: GSH was successfully entrapped into the Ginspo nanoparticles by hot melt method. The particle size of the Ginspo nanoparticles was 83.68±1.16 nm, and the surface charge was ?20.6±3.27 mV. Considering that the glutathione is a hydrophilic material, the entrapment efficiency was expected to be very low. Interestingly, however, it was 77.2±2.88% which was extraordinarily high. When the GSH- enrapped Ginspo nanoparticles were administered at a dose of 1 mg/Kg to a healthy volunteer, baseline GSH level before administration was 74.81 ppm, and the level increased to 81.94 ppm at 15 min after administration. The GSH level gradually decreased to baseline level over an hour. Conclusion: Ginspo nanoparticle appears to be a useful option for the development of GSH formulations taken by oral route without resorting to injection formulas.